[OPTICAL REVIEW Vol. 21, No. 3 (2014) 333-338]
© 2014 The Japan Society of Applied Physics

Application of Wide-Field Optical Coherence Tomography to Monitoring of Viability of Rat Brain In Vivo

Manabu SATO1* and Izumi NISHIDATE2

1Graduate School of Science and Engineering, Yamagata University, Yonezawa, Yamagata 992-8510, Japan
2Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan

(Received October 9, 2013; Revised November 22, 2013; Accepted December 3, 2013)

We investigated the feasibility of OCT in monitoring the viability of the brain. It was confirmed that after an overdose of pentobarbital sodium salt for an euthanasia, the OCT signal intensity increased before cardiac arrest and finally became 2.7 times, and by periodically changing the tissue temperature from 20 to 32 ℃ in vivo, average correlation coefficients between the ratio of signal intensity (RSI) and temperature were determined to be -0.42 to -0.50. RSI reversibly changed with subsequent variations of temperatures and finally increased rapidly just before cardiac arrest. These results indicate that RSI could correspond to decreases in viability.

Key words: viability, brain, wide field, optical coherence tomography

*E-mail address: msato@yz.yamagata-u.ac.jp